Figure 8
Ca2+ release from intracellular stores depends on ryanodine receptor activation. When ryanodine receptors were inhibited with ryanodine (40 µM), [Ca2+]i remained unaltered after the application of (A, B) 20:1 cafedrine/theodrenaline, (C, D) cafedrine alone, and (E, F) theodrenaline alone. The Ca2+-free control group was represented by the same 30 cells in each chart. FURA-2 340/380 ratio was normalized after a 100-s resting period, and each group consists of 30 cells from at least three different coverslips, ensuring independent measurements. Scale bar width represents 100 s. n = number of individual cells, ***p < 0.001, ns: not significant, Wilcoxon rank sum test was used to test ryanodine alone vs. drug preparation during inhibition, otherwise Mann–Whitney U test, adjusted α-level = 0.013. ⊥ SEM, box and whisker plots indicate median, interquartile range (box), minimum and maximum (whiskers).
