Figure 1

Schematic description of the KaScape process, not to scale. The random dsDNA pool (n = 4,5,6, or 7), represented by colored rectangular bars above, and the His-tagged TF DBD were prepared. The pooled dsDNAs consisted of approximately 30 base pairs with flanking sequences (Table S1). Next, \(2\times {10}^{-11}\) mol protein and \({10}^{-10}\) mol dsDNA were mixed in 2 mL buffer. The TF-DBD and dsDNAs were then incubated for 30 min. Magnetic His-tag purification beads were added, and rotation was performed for one hour, and the system was then washed and rotated 3 times. The dsDNA and TF-DBD complexes were then separated from the free unbound dsDNAs. Finally, the random dsDNA pool and bound dsDNAs were extended and used to produce the dsDNA library separately for next-generation sequencing.