Figure 4

AtWRKY1N binding specificity characterized by KaScape experiments with a series of dsDNA sequences of random base length. Upper figures are the binding energy landscape on a K-mer graph. Lower figures are the corresponding PWM sequence logo. The poorly informative positions are dropped. The sequences with relative binding energies of less than − 1 (also see the red line cutoff in Fig. 2c) are used to generate the PWM sequence logo. (a) The random base length of the sequences used in the KaScape experiment is 4. (b) The random base length of the sequences used in the KaScape experiment is 5. (c) The random base length of the sequences used in the KaScape experiment is 6. (d) The random base length of the sequences used in the KaScape experiment is 7.