Table 2 List of the potentially sensitive regions according to the cryoprotectant.

From: Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa

Regions

Nb

DMCs

DMSO

Nb

DMCs

MeOH

Nb

DMCs

Gly

Genomic

feature

Gene

Gene ID

2: 84,969,754–757

2

0

NA

Introns

NA

ENSOMYG00000033911

3: 28,999,827–911

0

0

4

Promoter 5kb

znf385d

ENSOMYG00000007065

3: 38,641,242–260

0

0

2

Intron 1

ptprub

ENSOMYG00000000944

6: 27,006,900–938

3

0

NA

Promoter 5kb

ebf2

ENSOMYG00000007222

6: 63,230,513–563

0

3

0

Introns

adam10a

ENSOMYG00000010593

9: 13,905,316–359

0

0

2

Exons

NA

ENSOMYG00000012701

9: 40,751,199–319

0

3

0

Promoter 5kb

NA

ENSOMYG00000007596

10: 58,822,172–181

2

NA

NA

Intron 1

NA

ENSOMYG00000026688;

ENSOMYG00000026627;

ENSOMYG00000026688;

ENSOMYG00000026627

14: 50,325,080–136

0

2

0

Intron 1

pcdhb

ENSOMYG00000014270

16: 13,278,338–342

NA

2

NA

Introns

SLC39A11

ENSOMYG00000033173

17: 57,694,790–858

0

2

0

Introns

cdh4

ENSOMYG00000044618

18: 26,437,868–930

NA

NA

7

Promoter 5kb

OSBPL10

ENSOMYG00000025071

19: 14,572,357–390

0

0

2

Exons; intron 1

ndufs8a

ENSOMYG00000005413

19: 43,252,495–511

0

0

2

Promoter 5kb

Ick

ENSOMYG00000032229

19: 45,634,077–080

NA

2

NA

Introns

col12a1a

ENSOMYG00000006853

20: 20,273–301

0

3

1

Downstream

5_8S

_rRNA

ENSOMYG00000008170

20: 3,482,719–759

3

NA

0

Introns

cdhr1

ENSOMYG00000009577

20: 13,286,267–314

2

0

1

Promoter 5kb

p4ha1a

ENSOMYG00000043711

20: 30,738,907–987

0

0

3

Introns

khdrbs2

ENSOMYG00000043413

25: 19,063,744–823

0

4

NA

Intron 1

slc26a2

ENSOMYG00000017229

25: 82,566,468–493

NA

1

2

Introns

smoc1

ENSOMYG00000037447

29: 15,383,450–576

NA

11

0

Exon 1

NA

ENSOMYG00000042159

MSJN01,019,798.1:

257,543–574

NA

0

2

Intron 1

NA

ENSOMYG00000040507

MSJN01,053,808.1:

39,504–747

0

0

8

Introns

NA

ENSOMYG00000030867

MSJN01,068,470.1:

831–873

0

2

0

Exon 1; Promoter 5kb

U2

ENSOMYG00000041560;

ENSOMYG00000041563

MT: 6657–6754

0

2

0

*:See legend

  1. The sequenced regions which displayed at least 2 DMCs (differentially methylated cytosines) between fresh and cryopreserved samples within 100 b (read size) in regions others than intergenic were included in this list. Gly: glycerol. Regions: chromosome number and region coordinates on the reference genome. Gene: name from the ensembl database (version 104) according to the reference genome (Omyk_1.0 genome version). Promoter 5 kb: region 5 kb upstream of the transcription starting site; Exon 1: first exon; Downstream: region 1 kb downstream of the transcription end site; NA: not available. *the 2 DMCs belonged to the exon1 of cox1, downstream of nd2, promoter 5 kb of cox2, promoter 5kb of atp8, promoter 5kb of atp6, promoter 5kb of cox3, promoter 5kb of nd3, promoter 5kb of nd4l, promoter 5kb of nd4.