Figure 6
Functional response of muscarinic M2 receptors. GTPγS binding to Gi1 (upper left), Gi2 (upper middle), Gi3 (upper right) GoA (lower left) and GoB (lower right) G-proteins upon stimulation of M2 muscarinic receptors by carbachol (red), iperoxo (green), NDMC (blue) or oxotremorine (yellow) at the concentration indicated on the x-axis is expressed as the fraction of maximal response of the system (EMAX). Data are means ± SD from 3 to 5 independent experiments performed in quadruplicates. Solid curves, Black & Leff equation (Eq. (7)). Dotted curves, Hill equation (Eq, (11)). Parameter estimates are in Supplementary Information Table S2.
