Figure 2 | Scientific Reports

Figure 2

From: Chlorogenic acid protects against myocardial ischemia–reperfusion injury in mice by inhibiting Lnc Neat1/NLRP3 inflammasome-mediated pyroptosis

Figure 2

CGA pretreatment decreases the expression levels of Lnc Neat1 and inhibits the activation of the NLRP3 inflammasome in MIRI mice. (a) CGA pretreatment reduced the I/R injury-induced expression levels of Lnc Neat1 in myocardial tissue.The expression levels of Lnc Neat1 were measured by qRT-PCR. (b) CGA pretreatment decreased the I/R injury-induced mRNA expression levels of NLRP3 and ASC. The mRNA expression levels of NLRP3 and ASC were measured by qRT-PCR. (c,d) CGA pretreatment lowered the I/R injury-induced protein expression levels of NLRP3 in myocardial tissue. The protein expression levels of NLRP3 were measured by Western blotting analysis. (e) CGA pretreatment markedly inhibited I/R injury-mediated ASC oligomerization in mouse myocardial tissue. ASC oligomerization was measuring by Western blotting analysis. (f,g) CGA pretreatment decreased the I/R injury-induced protein expression of NLRP3 and ASC in the cytoplasm of cardiomyocytes. Immunohistochemistry was used to measure the protein expression levels of NLRP3 and ASC in the cytoplasm of cardiomyocytes. Representative images were captured using an optical microscope (×400  magnification). The data are expressed as the mean ± SD. n = 15 mice in each group. The full-length blots are presented in Supplementary Fig. 1. *P < 0.05 vs. the sham group, #P < 0.05 vs. the I/R group.

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