Figure 1
From: An in vitro model and the underlying pathways of sinonasal inverted papilloma development
Transgene expression level in transduced iNP2 cells. (A) Expression levels of EGFR as well as vinculin as a loading control in iNP2 cells and those transduced with mutant EGFR were examined in triplicate by western blotting. To expose one blot to several antibodies at the same time, the membrane was cut into several pieces at desired positions prior to exposure to specific antibodies. The grouping of blots in (A) was cropped from the same samples loaded in 1 gel. The original western blot images of all experimental replicates were presented in the supplementary Fig. S1. (B) Band intensities of EGFR (normalized to those of vinculin) were quantified and indicated as bar graphs. “a” indicate statistically significant differences (a: p < 0.05).
