Figure 4 | Scientific Reports

Figure 4

From: Hypersensitivity of myelinated A-fibers via toll-like receptor 5 promotes mechanical allodynia in tenascin-X-deficient mice associated with Ehlers–Danlos syndrome

Figure 4

Effects of pharmacological sensory fiber-specific silencing on the responses of sensory afferent fibers using transcutaneous sine wave stimuli in Tnxb−/− mice. The current threshold represents the minimum intensity (μA) required to produce a paw withdrawal response with sine wave electrical stimulation at 5 Hz (a), 250 Hz (b), and 2000 Hz (c) in mice at the indicated time after i.pl. administration of vehicle, QX-314 (30 mM) plus flagellin (0.5 μg), and QX-314 (30 mM) plus capsaicin (10 μg). Vehicle (n = 6), QX-314 (n = 11), QX-314 + flagellin (n = 13), and QX-314 + capsaicin (n = 10). ***P < 0.001, **P < 0.01, *P < 0.05 versus the vehicle-treated value, two-way repeated measurement ANOVA revealed a significant treatment (5 Hz: F(3,36) = 7.007, P = 0.0008; 250 Hz: F(3,36) = 4.872, P = 0.0060; 2000 Hz: F(3,36) = 6.524, P = 0.0012) with post hoc Bonferroni’s multiple comparison test. The data are expressed as the mean ± S.E.M.

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