Figure 4 | Scientific Reports

Figure 4

From: Investigating the role of aae-miR-34-5p in the regulation of juvenile hormone biosynthesis genes in the mosquito Aedes aegypti

Figure 4

The expression patterns of miR-34 and three JH biosynthesis genes during mosquito development. Individual female pupae were collected 2 (− 2d) and 1 (− 1) day prior to adult emergence, and 1, 2, 3 and 4 days after adult emergence (1d–4d). The extracted total RNA was then subjected to RT-qPCR. (A) The relative expression levels of miR-34 during the developmental stages. (B–D) The relative expression levels of three genes involved in JH biosynthesis (see Fig. 1A). Each data point represents a single mosquito. One-way ANOVA was used to compare the treatments. The error bars in all the graphs represent standard error of mean (SEM) of biological replicates each represented by a data point. **, p < 0.01; ****, p < 0.0001.

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