Figure 4 | Scientific Reports

Figure 4

From: Significant influence of four highly conserved amino-acids in lipochaperon-active sHsps on the structure and functions of the Lo18 protein

Figure 4

Measuring the impact of point modifications on the structure of proteins. In silico prediction of secondary structure of the modified protein by PredictPortein (A)Structure modification of Lo18 (black), E60K (red), T79V (yellow), G82V (green) and R99D (blue) induced by thermal ramping (25 °C to 50 °C on the four types of secondary structures, (B) α-helix, (C) β-sheet, (D) turns and (E) other secondary structures, evaluated by SRCD. The data represent the means and SE of three independent experiments. (F) Prediction of the impact of point mutation substitution on secondary structure accessibility. (G) Oligomeric structure of Lo18 WT or modified. Immunolabelling of Lo18 after in vivo cross-linking. The diamond-shaped numbers from 1 to 3 indicate the presence of monomer, dimer and trimer respectively.

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