Table 1 SRCD measurement of secondary structure (α-helix, β-sheet, turn and other structures) of Lo18 or modified proteins.
a-Helix (%)/ Temperature (°C) | 25 | 35 | 45 | 55 | 60 |
---|---|---|---|---|---|
Lo18 | 1.5 ± 0.4A | 2.6 ± 0.2A | 2.7 ± 0.5A | 3.2 ± 0.2A | 3.8 ± 0.9A |
E60K | 31.2 ± 1.6C | 26.1 ± 1.2C | 15.9 ± 7.8BC | 11.7 ± 2.0B | 13.8 ± 5.5ABC |
G82V | 5.9 ± 7.3AB | 10.1 ± 7.1AB | 7.3 ± 5.8AB | 1.9 ± 1.7A | 6.1 ± 10.6AB |
T79V | 14.1 ± 6.2B | 15.1 ± 5.2B | 10.9 ± 4.9B | 20.1 ± 10.2BC | 20.2 ± 5.5BC |
R99D | 28.4 ± 1.8C | 25.1 ± 1.3C | 23.9 ± 3.5C | 27.7 ± 5.6C | 25.2 ± 6.0C |
3-sheet (%)/Temperature (°C) | 25 | 35 | 45 | 55 | 60 |
---|---|---|---|---|---|
Lo18 | 42.8 ± 3.2A | 43.1 ± 4.0A | 41.9 ± 1.7A | 30.9 ± 1.9A | 32.3 ± 2.2A |
E60K | 12.3 ± 1.8B | 14.6 ± 1.3B | 29.5 ± 3.7B | 31.1 ± 2.9A | 31.5 ± 2.5A |
G82V | 29.7 ± 7.8C | 25.9 ± 6.1C | 29.7 ± 5.4B | 34.6 ± 5.3A | 29.2 ± 12.8A |
T79V | 28.6 ± 7.4C | 27.0 ± 6.7C | 25.5 ± 1.8BC | 18.9 ± 5.3B | 13.5 ± 4.6B |
R99D | 17.6 ± 2.2D | 18.2 ± 0.8D | 19.2 ± 5.9C | 17.2 ± 1.9B | 17.5 ± 2.2AB |
Turn (%)/ Temperature (°C) | 25 | 35 | 45 | 55 | 60 |
---|---|---|---|---|---|
Lo18 | 12.6 ± 1.3A | 12.1 ± 1.9A | 12.8 ± 1.2A | 17.1 ± 0.7A | 15.9 ± 1.4A |
E60K | 14.1 ± 1.1A | 12.9 ± 1.3AB | 10.5 ± 1.4B | 9.7 ± 0.9C | 10.6 ± 2.5A |
G82V | 17.3 ± 6.0A | 20.5 ± 3.4C | 19.8 ± 2.0C | 14.2 ± 3.8B | 16.5 ± 5.8A |
T79V | 13.2 ± 2.3A | 11.7 ± 0.9A | 11.1 ± 1.0B | 14.5 ± 2.2B | 15.9 ± 3.2A |
R99D | 15.2 ± 1.0A | 14.8 ± 0.9BC | 16.0 ± 1.4C | 19.1 ± 1.1A | 19.6 ± 0.3A |
Other structure (%)/Temperature (°C) | 25 | 35 | 45 | 55 | 60 |
---|---|---|---|---|---|
Lo18 | 43.1 ± 2.1A | 42.2 ± 2.3A | 42.7 ± 1.1A | 48.8 ± 1.5A | 48.0 ± 0.4A |
E60K | 42.3 ± 2.0A | 46.3 ± 1.7A | 44.1 ± 5.7A | 47.6 ± 2.0A | 44.1 ± 5.4A |
G82V | 47.1 ± 5.6A | 43.5 ± 4.8A | 43.2 ± 2.3A | 49.3 ± 0.2A | 48.2 ± 4.0A |
T79V | 44.1 ± 1.7A | 46.2 ± 2.8A | 52.5 ± 6.2A | 46.5 ± 13.0A | 50.4 ± 5.0A |
R99D | 38.8 ± 3.0A | 41.9 ± 2.0A | 40.9 ± 1.4A | 36.0 ± 4.8A | 37.8 ± 4.0A |