Table 1 SRCD measurement of secondary structure (α-helix, β-sheet, turn and other structures) of Lo18 or modified proteins.

From: Significant influence of four highly conserved amino-acids in lipochaperon-active sHsps on the structure and functions of the Lo18 protein

a-Helix (%)/ Temperature (°C)

25

35

45

55

60

Lo18

1.5 ± 0.4A

2.6 ± 0.2A

2.7 ± 0.5A

3.2 ± 0.2A

3.8 ± 0.9A

E60K

31.2 ± 1.6C

26.1 ± 1.2C

15.9 ± 7.8BC

11.7 ± 2.0B

13.8 ± 5.5ABC

G82V

5.9 ± 7.3AB

10.1 ± 7.1AB

7.3 ± 5.8AB

1.9 ± 1.7A

6.1 ± 10.6AB

T79V

14.1 ± 6.2B

15.1 ± 5.2B

10.9 ± 4.9B

20.1 ± 10.2BC

20.2 ± 5.5BC

R99D

28.4 ± 1.8C

25.1 ± 1.3C

23.9 ± 3.5C

27.7 ± 5.6C

25.2 ± 6.0C

3-sheet (%)/Temperature (°C)

25

35

45

55

60

Lo18

42.8 ± 3.2A

43.1 ± 4.0A

41.9 ± 1.7A

30.9 ± 1.9A

32.3 ± 2.2A

E60K

12.3 ± 1.8B

14.6 ± 1.3B

29.5 ± 3.7B

31.1 ± 2.9A

31.5 ± 2.5A

G82V

29.7 ± 7.8C

25.9 ± 6.1C

29.7 ± 5.4B

34.6 ± 5.3A

29.2 ± 12.8A

T79V

28.6 ± 7.4C

27.0 ± 6.7C

25.5 ± 1.8BC

18.9 ± 5.3B

13.5 ± 4.6B

R99D

17.6 ± 2.2D

18.2 ± 0.8D

19.2 ± 5.9C

17.2 ± 1.9B

17.5 ± 2.2AB

Turn (%)/ Temperature (°C)

25

35

45

55

60

Lo18

12.6 ± 1.3A

12.1 ± 1.9A

12.8 ± 1.2A

17.1 ± 0.7A

15.9 ± 1.4A

E60K

14.1 ± 1.1A

12.9 ± 1.3AB

10.5 ± 1.4B

9.7 ± 0.9C

10.6 ± 2.5A

G82V

17.3 ± 6.0A

20.5 ± 3.4C

19.8 ± 2.0C

14.2 ± 3.8B

16.5 ± 5.8A

T79V

13.2 ± 2.3A

11.7 ± 0.9A

11.1 ± 1.0B

14.5 ± 2.2B

15.9 ± 3.2A

R99D

15.2 ± 1.0A

14.8 ± 0.9BC

16.0 ± 1.4C

19.1 ± 1.1A

19.6 ± 0.3A

Other structure (%)/Temperature (°C)

25

35

45

55

60

Lo18

43.1 ± 2.1A

42.2 ± 2.3A

42.7 ± 1.1A

48.8 ± 1.5A

48.0 ± 0.4A

E60K

42.3 ± 2.0A

46.3 ± 1.7A

44.1 ± 5.7A

47.6 ± 2.0A

44.1 ± 5.4A

G82V

47.1 ± 5.6A

43.5 ± 4.8A

43.2 ± 2.3A

49.3 ± 0.2A

48.2 ± 4.0A

T79V

44.1 ± 1.7A

46.2 ± 2.8A

52.5 ± 6.2A

46.5 ± 13.0A

50.4 ± 5.0A

R99D

38.8 ± 3.0A

41.9 ± 2.0A

40.9 ± 1.4A

36.0 ± 4.8A

37.8 ± 4.0A

  1. Data corresponding to means ± SE and statistical analysis. Measurement were replicat three time and analyse by Kruskal–Wallis statistical test (P-value < 0.05).