Figure 1
From: Expression of G protein-coupled receptor GPR19 in normal and neoplastic human tissues
Verification of the specificity of the anti-GPR19 antibody by immunocytochemistry analyses. (A) OH-1 cells or (D) NCI-h82 cells expressing GPR19 endogenously were fixed and stained with the anti-GPR19 antibody, followed by an Alexa Fluor 488-conjugated anti-rabbit secondary antibody. (B, E) For analysis of the specificity of the antibody, GPR19 expression was silenced in OH-1 cells or NCI-h82 cells using a GPR19-specific siRNA. (C, F) For adsorption controls, the anti-GPR19 antibody was preincubated for 2 h with 10 µg/ml of the peptide used for immunisations of the rabbits. Green, GPR19 immunosignal; blue, 4′,6-diamidino-2-phenylindole (DAPI) staining of DNA. Scale bar, 50 µm (A–F).
