Figure 2
From: Expression of G protein-coupled receptor GPR19 in normal and neoplastic human tissues
Verification of the specificity of the anti-GPR19 antibody by Western blot analyses. Left lane: Western blot analysis of membrane preparations of OH-1 cells that endogenously express GPR19 (“control”). Middle lane: Western blot analysis of membrane preparations of OH-1 cells after transfection with siRNA targeting GPR19 (“+ siRNA”). Right lane: For adsorption control, the antibody was preincubated for 2 h with 10 µg/ml of the immunising peptide (“+ peptide”). Ladder indicates migration of protein molecular weight markers (kDa). All results are representative of three independent experiments.
