Figure 7 | Scientific Reports

Figure 7

From: Treatment with a Lactococcus lactis that chromosomally express E. coli cfaI mitigates salivary flow loss in a Sjögren’s syndrome-like disease

Figure 7

Long-term oral treatment with LL 301 reduces the number of Tfh cells in the SMGs and proinflammatory cytokines. SMGs were isolated from the same mice used in Fig. 6. Upon study termination at 31 weeks of age (one week after the last dose), SMGs were examined levels of (A–E) Tfh cells by flow cytometry analysis. Flow cytometry plots representative of SMG Tfh cells are shown for (A) PBS- and (B) LL 301-treated mice. The percentage of SMG (C) CXCR5+, (D) BCL-6+, and (E) CXCR5+ BCL-6+ CD4+ T cells are shown. (F) Flow cytometry analysis was also done for the detection of the percentage of Foxp3+ CD4+ T cells. (G) Purified SMG lymphocytes from PBS- and LL 301-treated females were stimulated with anti-CD3 and anti-CD28 mAbs for 4 days, and culture supernatants were analyzed for IL-6 production by cytokine-specific ELISA. Depicted are the means ± SEM; **P < 0.01 and *P < 0.05 versus PBS-dosed mice.

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