Figure 2
From: Superiority of systemic bleomycin to intradermal HOCl for the study of interstitial lung disease
Intradermal HOCl injections (6 weeks) did not impair lung function or induce lung fibrosis. (a) Mean pressure–volume (P–V) loops for PBS and HOCl-injected mice. (b) Static lung compliance (Cst) (n = 20–24). (c) Micrographs of H&E and MT—stained lung sections (low magnification and × 20 and × 40 magnification) (n = 11–15). Scale bar: 100 μm (× 20 magnification) and 50 μm (× 40 magnification). (d) Image analysis of PSR-stained slides (n = 7–8). (e) lung hydroxyproline content in PBS and HOCl-injected animals (n = 6). (f) relative gene expression of α-smooth muscle actin (ACTA2), (g) transforming growth factor β1 (TGF- β1), (h) collagen type I alpha 1 (Col1a1), (i) collagen type IV alpha 1 (Col4a1), and (j) fibronectin (FN) in lung tissue. Data are represented as mean ± SEM from 2 to 4 independent pooled experiments. Data were analyzed using Two-way ANOVA (a) and Mann–Whitney U test (b, d–j). ns not significant.
