Figure 6

HOCl injections induce comparable skin fibrosis in Nrf2^−/− and WT control animals. (a) Skin thickness in mice increases over the course of 6 weeks of HOCl injections (n = 4–6). (b) Average measurements of dermal thickness (n = 4–6). Representative images of (c) H&E and (d) PSR staining of Nrf2^−/− and C57 HOCl-treated mice. H&E sections of both Nrf2^−/− and C57 mice show epidermal hyperplasia and hyperkeratosis indicated by the black arrow (H&E staining, panels i) and loss of adipose tissue/appendages in certain areas with preservation of them in adjacent areas (H&E staining, panels ii). Black arrows in the PSR-stained sections show representative dermal measurements. Data are represented as mean ± SEM from 2 experiments. Data were analyzed using Two-way ANOVA (a) or Mann–Whitney U test (b). ns not significant.