Figure 1

ARPV and ARPV/ZIKV do not replicate in vertebrate cells (Vero-76) during coinfection. Growth kinetics for each virus were compared for coinfected and control groups of: (a) ARPV and ZIKV intracellular factions, (b) ARPV and ZIKV extracellular factions, (c) ARPV/ZIKV and ZIKV intracellular factions, and (d) ARPV/ZIKV and ZIKV extracellular factions. Vero-76 cells were infected at a target multiplicity of infection (MOI) of 0.1 with each virus, and intracellular and supernatant fractions were taken at 0, 3, 6, 12, 24, 36, 48, 72, 96, and 120 h post-infection (hpi). Viral RNA were extracted and quantified using RT-qPCR. Data points are presented as log₁₀ fold change in virus titer, where the fold change was estimated by dividing the virus titer at the indicated timepoint by the virus titer measured at the 0 h time point for that respective virus. Asterisks indicate significance compared via two-way ANOVA and mixed-effects analyses. Unless otherwise marked, asterisks indicate significance compared ZIKV control; p ≤ 0.033 (*).