Figure 5
From: Single-neuron mechanical perturbation evokes calcium plateaus that excite and modulate the network
Mechanical responses propagate through a defined neuronal ensemble. (a) Functional connections from calcium imaging of spontaneous activity prior to mechanical stimulation. (ai) Exemplary calcium traces of the target (T), responders (R), and non-responders (NR) before the target deformation. R/NR labelled after mechanical stimulus measurement. (aii) Box plot showing cross-correlation coefficients of spontaneous pre-stimulus calcium spikes, with whiskers spanning over 1.5 × IQR. ****: p ≤ 10–4, estimated via two-tailed Mann–Whitney test. (b) Direct connectivity estimations through paired whole-cell measurements following the deformation of the target. (bi) Schematic overview of different configurations; neurons responding to a neighbor’s deformation (top and middle) are in blue, non-responders are in gray. (bii) Neighbor’s responses to APs evoked via electrical stimulation. Rows match schematics depicted in (bi). (biii) Spontaneous activity detected intracellularly from two patched neurons. Biv. Focus on bursting events (yellow in (biii)) or individual post-synaptic potentials (dotted in (biii)).
