Figure 2 | Scientific Reports

Figure 2

From: Conversion of methylmercury into inorganic mercury via organomercurial lyase (MerB) activates autophagy and aggresome formation

Figure 2

Upregulation of LC3B and p62 genes in MeHg-exposed MerB-expressing cells. (a) Heat map of RT-qPCR data showing gene expression (mean fold change, n = 3/treatment group) in WT and MerB-expressing cells after 8 h of treatment with 4 µM of MeHg was created using R software (ver. 4.0.5, https://www.r-project.org/). All gene values were normalized to that of the housekeeping gene, GAPDH. The color scales indicate the log 2 ratio of relative expression levels. (b) WT and MerB-expressing cells were treated with 4 µM MeHg and harvested at the indicated times. LC3B and p62 mRNA levels were quantified by RT-qPCR. Data shown are the mean ± standard deviation (calculated from three independent experiments, each with two RT-qPCR replicates per condition; results are represented as the mean ± standard deviation values compared to DMSO-treated controls for each time point). Significant differences were determined by one-way ANOVA with multiple comparisons corrected by Dunnett test; ***p < 0.001, **p < 0.01.

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