Figure 6

Transient formation of large p62 and ubiquitin-positive aggregates in MeHg-exposed MerB-expressing cells. (a) WT and MerB-expressing cells were treated with 4 µM MeHg for the indicated periods, and then immunofluorescence staining was performed with p62 and ubiquitin antibodies. Scale bar, 20 µm. (b) Graphics outlining the scheme for measuring the dispersion distance of p62 in the Fig. 6c experiment. (c) Boxplot showing the dispersion distance of p62 from confocal images of MerB-expressing cells. n = 44 cells (MeHg for 8 h) and 44 cells (MeHg for 24 h). In the boxplot, the center line indicates the median, and box boundaries indicate the 25th and 75th percentiles; **, p < 0.01 (Bunner–Munzel test).