Figure 1 | Scientific Reports

Figure 1

From: An open-source, automated, and cost-effective platform for COVID-19 diagnosis and rapid portable genomic surveillance using nanopore sequencing

Figure 1

Clinical validation of MAVRICS protocols. RNA from 94 clinical samples collected in viral transport medium (VTM) was extracted in parallel using MAVRICS TRIzol, MAVRICS Bis–Tris, and MagMAX™ protocols. For SARS-CoV-2 diagnosis, SARS-CoV-2 ORF 1ab, S gene, and N gene and the internal control MS2 were detected by TaqPath™ COVID-19 Combo Kit. (A) Ct value (mean ± S.D.) for the targets in the three protocols, *p < 0.01 (paired Student’s t-test). (B) Correlation of Ct values of SARS-CoV-2 targets between tested protocols and corresponding Pearson correlation coefficient (r). The input sample was 200µl for MAVRICS-BisTris and MagMAX™ protocols and 100 µl for the MAVRICS-TRIzol protocol. The criteria for amplification curve, Ct value for each target, and SARS-CoV-2 sample detection were according to the TaqPath™ kit as described in "Methods".

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