Figure 3 | Scientific Reports

Figure 3

From: An open-source, automated, and cost-effective platform for COVID-19 diagnosis and rapid portable genomic surveillance using nanopore sequencing

Figure 3

Robotic extraction from swab clinical samples. Robotic MAVRICS RNA extraction was performed in clinical samples using a Tecan EVO-200 system, one-step RT-qPCR was performed with R3T RT-qPCR system and the nCoV_N1, nCoV_N2 and RNase P assays. (A and B) Ct values for RNase P, nCoV_N1 and nCoV_N2 obtained from the samples categorized as negative (A) and positive (B) for SARS-CoV-2. *: Ct value undetermined due to no amplification for most samples. (C and D) Correlation (C) and difference (D) of Ct values between robotic and manual extraction from eight positives samples for RNase P, nCoV_N1 and nCoV_N2. No significant difference was found (Student’s t-test).

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