Table 2 Output from RiboSplitter showing protein changes for 6 selected alternative splicing events.

From: Prediction of alternative pre-mRNA splicing outcomes

Gene

Event ID

Same start AA

AA different in isoform 1

AA different in isoform 2

Same end AA

CASP8

alt_5prime.24674

TLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAG

ALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCP

TVEPKREK*

 

PRKACB

alt_3prime.1668

VTDFGFAKRVKGRTWTLCGTPEYLAPEIILSK

TNQFRFMKRLFLE

GYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGK

 

DDX47

alt_3prime.9967

GVTDVLCEACDQLGWTKPTKIQIEAIPLALQ

 

GRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSGKCLRGKGS*

AVIVGGIDSMSQSLALAKKPHIII

RBM3

exon_skip.83079

LELPCPLKKESSSWEGSTLTPTSRHWKTTSAVSDLSLR

SLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSR

WSLSRTGRLSGPGVLVSSPSPTQSMLQLP*

 

USP14

intron_retention.27364

SDKKSSPQKEVKYEPFSFAD

 

GK*

DIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQ

KPNA5

intron_retention.47705

VSPCLNVLSRLLFSSDPDVLADVCWALSYLSDGPNDKIQAVIDSGVCRRLVELLM

 

*

HNDYKVVSPALRAVGNIVTGDDIQTQ

  1. Amino acid sequences (AA) that are the same at the start and end of isoforms 1 and 2, and sequences that are different in one isoform relative to the other. *Stop codon.
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