Figure 2 | Scientific Reports

Figure 2

From: Plant-expressed Zika virus envelope protein elicited protective immunity against the Zika virus in immunocompetent mice

Figure 2

Antibody responses elicited by ZikaEnv:hFc and ZikaEnv:aghFc in the presence of each adjuvant or an adjuvant combination. (A) Experimental strategies. C57BL/6 mice (n = 5) were immunized with ZikaEnv:hFc and ZikaEnv:aghFc with each adjuvant or with an adjuvant combination, or with PBS at weeks 0, 2, and 6. The adjuvants used are indicated in all figures. To measure the humoral immune response, blood samples were collected at weeks 0, 1, 4, and 7. (B, C) An indirect enzyme-linked immunosorbent assay (ELISA) was performed using ZikaEnv:hFc and ZikaEnv:aghFc as coating antigens. Serum samples were diluted at 1:100 and OD 450 values were measured using indirect ELISA. The data are presented as the mean ± SD of five mice per group. Statistical analysis: *P < 0.05, **P < 0.01, ***P < 0.001. (D) Virus neutralization titers against ZIKV, as determined using the plaque reduction neutralization test (PRNT) Mouse sera utilized for PRNT measurement were taken 7 weeks post immunization with each adjuvant, an adjuvant combination, or PBS.

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