Figure 3
Effects of protein synthesis or PKA inhibition on the effects induced by PDE4 inhibition in the DH after a short retrieval session. The experimental design is represented above the graphs. The red arrows represent the moment of treatment with ROF or VEH. The green arrow represents the moment of treatment with ANI or its VEH. The blue arrow represents the moment of treatment with H89 or its VEH. (A) No differences were observed among groups during retrieval and Test A1. However, animals that received ROF-VEH presented less freezing behavior during Test A2 when compared to the control (VEH-VEH) in the same session. Moreover, the group that received ROF-ANI, presented higher freezing behavior than the group that received ROF-VEH. n: VEH-VEH = 8; ROF-VEH = 7; ROF-ANI = 7; VEH-ANI = 7. (B) No differences were observed among groups during retrieval and Test A1. However, the animals treated with ROF-VEH presented less freezing behavior during Test A2 compared to the control (VEH-VEH) in the same session. Moreover, the group that received ROF-H89, presented higher freezing behavior than the group that received ROF-VEH. n: VEH-VEH = 9; ROF-VEH = 8; ROF-H89 = 9; VEH-H89 = 9. The data is represented by mean ± S.E.M. and the individual values of the percentage of freezing expressed by animals during each session. The * represents a significant difference (*P < 0.05) compared to the VEH-VEH group in the same session. The + represents a significant difference (+ P < 0.05) compared to the ROF-VEH group in the same session.
