Figure 4
Effects of PDE4 inhibition on total and phosphorylated CREB expression in the DH. The experimental design is represented above the graphs. The purple arrows represent the moment of treatment with ROF or VEH. (A–C) represent the samples collected 90 min after retrieval and treatment. (A) No changes were observed in CREB expression among groups. (B) The VEH and ROF groups presented higher pCREB expression than naive. C) The VEH and ROF groups presented a higher pCREB/CREB ratio than naïve; (n: NAIVE = 11; VEH = 13; ROF = 13). (D–F) represent the samples collected 90 min after Test A1. (D) The VEH and ROF groups presented higher CREB expression than naive. (E) The VEH group showed higher pCREB expression than naive. No differences were observed between ROF and naive or VEH groups. (F) No differences were detected in the pCREB/CREB ratio among groups. (n: NAIVE = 10; VEH = 11; ROF = 12). The data is represented by mean ± S.E.M. and the individual values of the percentage (compared to the naive group) of CREB or pCREB expression normalized for GAPDH or pCREB/CREB ratio. The # represents significant differences (#P < 0.05) compared to the naive group.
