Figure 5
Effects of PDE4 inhibition on the BDNF expression in the DH. The experimental design is represented above the graphs. The purple arrows represent the moment of treatment with ROF or VEH. (A–C) Represent the samples collected 90 min after retrieval and treatment. (A) The VEH and ROF groups presented lower pre-proBDNF than the naive group. (B) No differences were detected in proBDNF among groups. (C) No differences were detected in mBDNF among groups; (n: NAIVE = 10–11; VEH = 11–13; ROF = 11–13). (D–F) Represent the samples collected 90 min after Test A1. (D) No differences were detected in pre-proBDNF among groups. (E) The ROF-treated group showed higher proBDNF expression than the VEH-treated group. (F) No differences were detected in mBDNF expression among groups; (n: NAIVE = 10; VEH = 11; ROF = 12). The data is represented by mean ± S.E.M. and the individual values of the percentage (compared to the naive group) of pre-proBDNF, proBDNF or mBDNF expression normalized for GAPDH. The * represents a significant difference (*P < 0.05) compared to the VEH group. The # represents a significant difference (#P < 0.05) compared to the naive group.
