Figure 1
IGSF6 exhibits strong association with immune infiltration in colorectal cancer and could be a novel biomarker to evaluate immune infiltration. (A) In the TCGA database, a comprehensive analysis of the correlation between all genes and CD8+ T cell infiltration in pMMR colorectal cancer was performed using TIMER and Xcell algorithms. Among the top 500 genes, IGSF6 emerged as a highly ranked candidate. (B) Expression of IGSF in colorectal cancer from the GEPIA database. (C) Representative immunofluorescence images of IGSF6 expression in CRC and adjacent normal tissues from the patient. (D) Quantification of the score for IGSF6 level in tumor tissue versus adjacent tissue assessed by immunofluorescence assay. *p < 0.05, ***p < 0.001, ****p < 0.0001. p values were determined by paired-t test. (E) Correlation between immune cell infiltration and IGSF6 in pan-cancer, *p < 0.05, **p < 0.01, ***p < 0.001. (F) Correlation between immune cell infiltration and IGSF in CRC patients from TCGA database, n = 383. (G) Correlation between immune cell infiltration and IGSF in MMR-proficient CRC patients, n = 249. (H,I) Correlation between immune check points and IGSF6 expression in MMR-proficient patients by Spearman’s correlation coefficient. n = 249. (J) Correlation between TMB and IGSF6 expression in CRC patients by Spearman’s correlation coefficient, n = 359. (K,L) Unsupervised hierarchical clustering of colorectal tumors using ssGSEA scores for immune signatures identifies increasing levels of immune infiltrates. (J) from TCGA database, n = 367, (K) from GSE39582 database, n = 519.
