Figure 4
Effect of HIF-1α stabilization on LDHA, PDK1, and MCT4 protein expression. (a) LDHA protein was significantly reduced (F(2,8) = 38.55, p < 0.0001) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed a significant reduction in LDHA in 2- week (p = 0.0006) and 4-week Roxa (p < 0.0001) retinas compared to control retinas. (b) PDK1 protein was significantly higher (F(2,9) = 42.73, p < 0.0001) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed a significant PDK1 increase in 4 weeks compared to control (p = 0.0004) and 2-week Roxa (p < 0.0001) retinas. (c) MCT4 protein was significantly lower (F(2,9) = 13.64, p = 0.0019) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed MCT4 decreased in 4 weeks compared to control (p = 0.0064) and 2-week Roxa (p = 0.0023) retinas. (d) Representative immunofluorescence images showing MCT-4 (magenta) and GLAST (green) expression in retina cross-sections for the Control, Roxa 2-week, and Roxa 4-week groups. Scale bar = 20 µm. GCL = ganglion cell layer; INL = inner nuclear layer. Proteins were analyzed by capillary electrophoresis and normalized to total protein levels. Values shown are mean ± SEM of n = 3–4 per group.
