Figure 1 | Scientific Reports

Figure 1

From: Platelet-activating factor and protease-activated receptor 2 cooperate to promote neutrophil recruitment and lung inflammation through nuclear factor-kappa B transactivation

Figure 1

PAR2 blockade and inhibition of proteases impair PAF-induced neutrophil migration in BALB/c mice. (A,B) Mice were instilled intranasally with PBS or C-PAF. The number of infiltrating neutrophils was counted in BALF 4, 24, or 48 h after challenge. (C–G) Mice were pretreated with an intraperitoneal injection of WEB 2086 or ENMD1068, or with intranasal instillation of PBS or aprotinin 1 h before intranasal instillation of C-PAF or SLIGRL-NH2. The number of infiltrating neutrophils was counted in BALF 24 h after challenge. The results are representative of three independent experiments and are expressed as mean ± SEM from six mice per group. Statistical analysis was assessed by ANOVA followed by Newman-Keuls test, A, B and F: ***p < 0.001 and *p < 0.05 compared to control group (PBS); C, D, E and G: ***p < 0.001, **p < 0.01 and *p < 0.05 compared to C-PAF group; C, D, E and F: #p < 0.001 or ##p < 0.01 compared to control group (PBS).

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