Figure 2 | Scientific Reports

Figure 2

From: Platelet-activating factor and protease-activated receptor 2 cooperate to promote neutrophil recruitment and lung inflammation through nuclear factor-kappa B transactivation

Figure 2

PAR2 blockade impairs rolling and adhesion of leukocytes in the mesentery microcirculation of mice. (A–F) Representative imaging of mesentery microcirculation by intravital microscopy (neutrophils in red, anti-Ly6G/Ly6C-Gr-1; 0.2 mg/mL, i.v.). Mice were pretreated with ENMD1068 (0.5 mg/kg, i.p.) 1 h before stimulation with C-PAF (10−7 M, i.p.). After 4 h, rolling (G), velocity (H), and adhesion (I) of leukocytes (white arrows) were evaluated in the venules of mesentery microcirculation. The fluorescence intensity was monitored for 10 min using an objective Plan Apo 20 × calibrated by each venule. The results are representative of three independent experiments with similar results and are expressed as mean ± SEM from six mice per group. Statistical analysis was assessed by ANOVA followed by Newman-Keuls test, (G–I) ***p < 0.001 compared to C-PAF group and #p < 0.001 compared to control group (PBS). Scale bar: 90 μm or 43 μm.

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