Figure 4 | Scientific Reports

Figure 4

From: Platelet-activating factor and protease-activated receptor 2 cooperate to promote neutrophil recruitment and lung inflammation through nuclear factor-kappa B transactivation

Figure 4

PAFR/PAR2 physical interaction in RAW 264.7 murine macrophages. Cells were stimulated with C-PAF (100 nM), SLIGRL-NH2 (50 μM), or simultaneous co-stimulation (C-PAF 100 nM + SLIGRL-NH2 50 μM) for 20 min before addition of RIPA. Cell lysates were subjected to immunoprecipitation (A) and west immunoblotting (B) using antibodies to PAFR and PAR2. Representative image of the interaction and quantification of PAR2 and PAFR proteins immunoprecipitated. The experiments were performed in duplicate and one membrane was developed with anti-PAR2 and the other with anti-PAFR for normalization control. In data shown, each band in representative membrane image of western blotting correspond of three separate experiments realized before gel electrophoresis running. Values are expressed as mean ± SEM. Statistical analysis was assessed by ANOVA followed by Newman-Keuls test, B: *p < 0.05 compared to control group (PBS).

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