Figure 6 | Scientific Reports

Figure 6

From: Platelet-activating factor and protease-activated receptor 2 cooperate to promote neutrophil recruitment and lung inflammation through nuclear factor-kappa B transactivation

Figure 6

Expression of PAR2 and NF-КB (p65) transcription factor in RAW 264.7 cells. Cells were pretreated with ENMD1068 (5 μM) 1 h prior to C-PAF (100 nM) stimulation, fluorescence intensity was evaluated 4 h later. (A) Representative images of PAR2 (red), NF-kB (p65, green) and DAPI (nucleus, blue) expression. (B) PAR2 fluorescence intensity. (C) Nuclear fluorescence intensity for NF-κB (p65). (D) Cells were pretreated with ENMD1068 (5 μM) 1 h prior C-PAF (100 nM) stimulation. PAR2 mRNA was analyzed 1, 2, and 3 h later by qPCR. (B,C) Bars represent mean ± SEM (n = 3 preparations with 30 cells per group). Statistical analysis was by ANOVA followed by Newman-Keuls test, (B) and (D) ***p < 0.001 and *p < 0.05 compared to control group (PBS); (C) and (D) ***p < 0.001 and *p < 0.05 compared to C-PAF group and #p < 0.001 compared to control group (PBS).

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