Figure 5

Alignment of structures similar to GLT8D1 using the PROMALS3D server. The full length AlphaFold model of GLT8D1 was used to search the Dali²⁷ database (Fig. 4) and the structures of the top ten scoring proteins were aligned with the AlphaFold structure of GLT8D1(UniProt Q68CQ7) and structures of Klebsiella pneumoniae WbbM (PDB 6U4B), Streptococcus pneumoniae GlyE (PDB 5GVV), Homo sapiens LARGE-1 (PDB 7UI7), Chaetomium thermophilum UGGT (PDB 5N2J), Anaerococcus prevotii GT8 protein (PDB 3TXZT), Oryctolagus cuniculus Glycogenin-1 (PDB 1LLO), Mus musculus XXYLT1 (PDB 4WLG), Homo sapiens ABO (PDB 3V0Q), Chlorella virus UDP-Glc GlcT (PDB 2P73), and Arabidopsis thaliana XXT1 (PDB 6BSU) using PROMALS 3D²⁸. Conserved secondary structure elements are indicated as helices (h) or beta strands (e) and consensus amino acid positions are classified by amino acid character (aliphatic (l), aromatic (@), hydrophobic (h), alcohol (o), polar (p), tiny (t), small (s), bulky (b), positively charged (+), negatively charged (−), and charged (c)) or as bold uppercase for conserved. Landmark GT-A fold protein motifs include the DxD motif (green box), G-loop (purple box), divergent sequences of the xED motif that are not conserved in retaining GT8 enzymes (black dotted box, and C-term His (red box). The single disulfide bond predicted in the GLT8D1 structure is indicated by the yellow bracket. Three Asn-linked glycan sequons in GLT8D1 are indicated by gray boxes.