Figure 3 | Scientific Reports

Figure 3

From: Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence

Figure 3

Δ5msr mutant is highly susceptible to HOCl and ChT. Δ5msr mutant and S. Typhimurium strains were grown up to mid-log phase in LB broth and incubated with the indicated concentrations of HOCl or ChT, either directly adding to culture media (a, c), or after pelleting and suspending cultures in PBS (b, d). Following incubation of two hours (a, c) or 30 min (b, d), the cultures were serially diluted and plated on agar media. CFUs were enumerated following overnight incubation of the plates. Data are presented as mean ± SE (n = 8) for (a), (n = 9) for (b), (n = 3) for (c) and (n = 8) for (d) Data were analyzed by two-way ANOVA. ****p < 0.0001.

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