Figure 1 | Scientific Reports

Figure 1

From: A novel biomarker of MMP-cleaved cartilage intermediate layer protein-1 is elevated in patients with rheumatoid arthritis, ankylosing spondylitis and osteoarthritis

Figure 1

Alignment and specificity of the CILP-M assay. (A) Sequence alignment of the targeted sequence for CILP-M in human with mouse, bovine, and rat. Sequence is highlighted in yellow, and protease cleavage is marked with an arrow. (B) Specificity of the CILP-M assay. Reactivity towards the standard peptide (SLNPDTGLWE), truncated peptide (NPDTGLWE), elongated peptide (WSLNPDTGLWE) and non-sense standard peptide and coater (DSGPEYADVV). Signals are shown as relative luminescence (RLU) per second, as a function of standard peptide. Abbreviations: LLOQ, lower limit of quantification; ULOQ, upper limit of quantification.

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