Figure 2 | Scientific Reports

Figure 2

From: Bayesian posterior density estimation reveals degeneracy in three-dimensional multiple emitter localization

Figure 2

Two emitter separability using an astigmatic PSF, collecting 100 simulated frames into one reconstruction while varying emitter distance. (Columns 1 and 4) Example frames. (Columns 2 and 5) Zoomed in XY plane reconstruction. (Columns 3 and 6) Zoomed in XZ plane reconstruction. Emitters were placed in focal plane, with an intensity of 2000 photons each and a background of 20 photons. The ROI is 20 by 20 pixels, scalebars assume an effective pixel size of 100 nm. The reconstructed image consists of histograms from MCMC chains which used the MAP number of emitters as model.

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