Table 1 Single grain dry mass, grain yield per ear, total quantity of N per grain (Ntot), grain N yield per ear, and grain protein concentration at maturity (900 and 1050 °C days) for the null segregant (NS) and SPA RNAi (RNAi) lines under low (N−) and high (N+) nitrogen availability.

From: Storage protein activator controls grain protein accumulation in bread wheat in a nitrogen dependent manner

Variable

Treatment

N−

N+ 

NS

RNAi

NS

RNAi

Single grain dry mass (mg DM grain−1)

40.0 ± 0.5

41.2 ± 0.8

40.9 ± 0.6

42.6 ± 0.7

Grain yield per ear (g DM ear−1)

0.96 ± 0.03

1.05 ± 0.05

1.04 ± 0.02

1.14 ± 0.04*

Grain N (mg N grain−1)

0.92 ± 0.02

0.86 ± 0.01**

1.24 ± 0.02

1.27 ± 0.03

Grain N yield per ear (mg N ear−1)

22.4 ± 0.5

22.3 ± 0.8

32.0 ± 0.8

35 ± 1.3

Grain protein concentration (% of DM)

13.2 ± 0.2

11.9 ± 0.2***

17.3 ± 0.2

17.0 ± 0.3

  1. Data are means ± 1 s.e. for n = 8 independent replicates. Within a single N treatment, * (P < 0.05), ** (P < 0.01) and *** (P < 0.001) indicate significant differences between values for NS and RNAi lines from an ANOVA followed by the Dunnett post-hoc test.
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