Table 1 Oligonucleotides used for DNA amplification mitochondrial cytochrome oxidase I (COI) and ribosomal RNA (16S) genes, and nuclear sequences of the non-transcribed spacer region (ITS2) and nuclear ribosomal RNA (28S).

From: Disjunct habitat of cryptic Terebellides (Annelida, Trichobranchidae) species shows a phylogenetic link between polychaetes from the White and the North Seas

Gene

Primers

Primer melting temperature, ºC

COI

LCO1490 (GGTCAACAAATCATAAAGATATTGG

HCO2198 (TAAACTTCAGGGTGACCAAAAAATCA)

50

16S

16SANNF (GCGGTATCCTGACCGTRCWAAGGTA) or

16SARL (CGCCTGTTTATCAAAAACAT)

16SBRH (CCGGTCTGAACTCAGATCACGT)

50

ITS2

ITS58SF (GAATTGCAGGACACATTGAAC)

ITS28SR (ATGCTTAAATTCAGCGGGT)

58

28S

28SC1 (ACCCGCTGAATTTAAGCAT)

28SD2 (TCCGTGTTTCAAGACGG)

58

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