Figure 1

Effects of different treatments on oxidative stress indexes of MC3T3-E1 cells. (A) ROS images were detected by fluorescence microscope, scale bar = 100 μm; (B) ROS was detected by flow cytometry; (C) The average fluorescence intensity of ROS was determined by flow cytometry; (D) Quantitative analysis of SOD activity; (E) Quantitative analysis of MDA content; (F) CCK8 cell activity detection; (G) JC-1 fluorescence quantitative analysis (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).