Figure 5 | Scientific Reports

Figure 5

From: Trichostatin A enhances the titanium rods osseointegration in osteoporotic rats by the inhibition of oxidative stress through activating the AKT/Nrf2 pathway

Figure 5

The effects of TSA on Nrf2 and the effects of different treatments on protein expression of MC3T3-E1 cells after adding titanium powder were detected by nuclear cytoplasmic separation. (A) Representative expression bands of Nrf2 in nucleus, cytoplasm and total Nrf2; (B) Histogram of relative expression levels of Nrf2 in nucleus, cytoplasm and total Nrf2; (C) Representative bands of osteogenesis-related proteins (OPN, Runx2, BMP2, and OPN), apoptosis-related proteins (Caspase3, Bcl2, Bax, and Cleaved Caspase3), and some pathway-related proteins (AKT, HO-1, and NQO1) in MC3T3-E1 cells after adding titanium powder; (D) Relative expression levels of related proteins in each group compared with NC group. Original blots are presented in Supplementary Panel B and C (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

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