Figure 2
Cell cycle reentry and G1/S transition of Müller glia after MNU- and MMS-induced retinal injury. (A) qRT-PCR analyses of cell cycle-related genes (Ccnd1, Ccne1, Ccne2, and Ccna2) after MNU and MMS treatments. Each bar represents the mean ± standard deviation (SD, n = 3) and the values expressed relative to the controls (Cont) after normalization to Gapdh levels. (B) Double immunofluorescence for Sox9 (green) and p-pRb (red) at day 1.5 after treatments with the alkylating agents. (C) The proportions of p-pRb-positive Müller glia. (D) Double immunofluorescence for Sox9 (green) and BrdU (red) at day 2 after treatments with the alkylating agents. (E) The proportions of BrdU-positive Müller glia. Each bar of the graphs represents the mean ± SD (n = 3). *p < 0.05, **p < 0.01 (comparisons with control). #p < 0.05, ##p < 0.01 (comparisons between treatments at the same stage). ONL outer nuclear layer, INL inner nuclear layer. Scale bar = 20µm.
