Figure 3
The time course of photoreceptor degeneration after treatment with alkylating agents. (A) qRT-PCR analyses of rod genes (Rho and Gnat1) and cone genes (Opn1sw and Opn1mw) after MNU and MMS treatments. (B) Immunofluorescence for RXRγ (green) in combination with TUNEL (red) at day 0.5 after treatment with alkylating agents. White arrowheads indicate double positive cells. PL photoreceptor layer, ONL outer nuclear layer. Scale bar = 20µm. (C) Quantification of surviving cone photoreceptors (RXRγ+, TUNEL−) at day 0.5 after MNU and MMS treatments. Each bar of the graphs represents the mean ± SD (n = 3). *p < 0.05, **p < 0.01 (comparisons with control). #p < 0.05, ##p < 0.01 (comparisons between treatments at the same stage).
