Figure 3 | Scientific Reports

Figure 3

From: Modelling bronchial epithelial-fibroblast cross-talk in idiopathic pulmonary fibrosis (IPF) using a human-derived in vitro air liquid interface (ALI) culture

Figure 3

Electrical and mRNA analysis of in vitro cultures. Transepithelial Electrical resistance (TEER) measurements of in vitro cell cultures at day 35 of culture (A). Changes in gene expression of co-cultures when normalised and presented as fold log change to epithelial monocultures (B) or respective fibroblast monocultures (C). Comparisons were performed using a one-way ANOVA with Tukey’s multiple comparisons (****, P < 0.0001; ***, P = 0.0002;**, P = 0.002; *, P = 0.033). n = 2 donors for NHBEs, n = 3 donors for NHLFs and n = 3 donors for DHLFs, N = 4–8 for each condition and presented as the mean ± SD. NHBE = Normal Human Bronchial Epithelial cell; NHLF = Normal Human Lung Fibroblast; DHLF = Diseased Human Lung Fibroblasts.

Back to article page