Figure 4 | Scientific Reports

Figure 4

From: Modelling bronchial epithelial-fibroblast cross-talk in idiopathic pulmonary fibrosis (IPF) using a human-derived in vitro air liquid interface (ALI) culture

Figure 4

Representative brightfield images of the apical surface of untreated VS TGFβ-1 treated cultures (A). Scale bars = 1 mm, inset =  × 3 magnification. Quantification of apical surface contrast (B), as a proxy for mucus reflectance, for TGFβ-1 treated cultures. Changes in TEER post-stimulation (C) when normalised and presented as percentage change from untreated. Comparisons were performed using multiple T tests with Sidak-Holm multiple comparisons (****, P < 0.0001; ***, P = 0.0002; **, P = 0.002; *, P = 0.033). n = 2 donors for NHBEs, n = 3 donors for NHLFs and n = 3 donors for DHLFs, N = 4–8 for each condition and presented as the mean ± SD. NHBE = Normal Human Bronchial Epithelial cell; NHLF = Normal Human Lung Fibroblast; DHLF = Diseased Human Lung Fibroblasts ; UT = untreated.

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