Table 2 List of analytes and typical detection limits used for ELISA.

From: Modelling bronchial epithelial-fibroblast cross-talk in idiopathic pulmonary fibrosis (IPF) using a human-derived in vitro air liquid interface (ALI) culture

Analyte

LLOD

LLOQ

ULOQ

Dynamic range

Unit

IFN-γ

0.37

1.76

938

0.37–938

pg/mL

IL-1β

0.05

0.646

375

0.05–375

pg/mL

IL-2

0.09

0.89

938

0.09–938

pg/mL

IL-4

0.02

0.218

158

0.02–158

pg/mL

IL-6

0.06

0.633

488

0.06–488

pg/mL

IL-8

0.07

0.591

375

0.07–375

pg/mL

IL-10

0.04

0.298

233

0.04–233

pg/mL

IL-12

0.11

1.22

315

0.11–315

pg/mL

IL-13

0.24

4.21

353

0.24–353

pg/mL

TNF-α

0.04

0.69

248

0.04–248

pg/mL

  1. *Taken from MSD ® MULTI_SPOT assay system proinflammatory panel 1 human kit manual.
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