Figure 4
From: Hyperoxia prevents the dynamic neonatal increases in lung mesenchymal cell diversity
Mural cells encompass proliferating pericytes at P7 and harmonized vascular smooth muscle characterized by specific transcription factors. (A) Mural cell type composition between E18.5 and P21. Colors as in Fig. 2A. (B) Fraction of proliferating pericytes at postnatal time points. (C) Representative FISH image of Mki67+ Cox4i2+ proliferating pericytes in P7 lungs. (D) Embedding of pericytes, colored by time point. (D) Violin plots of the expression of select genes that change in expression over perinatal development in pericytes. (F) Embedding of VSM, colored by time point. (G) Violin plots of gene expression in pericytes versus VSM (left) and ASM versus VSM (right) for transcription factors (TFs) marking either cell type. (H) Combined statistical enrichment of expressed transcription factors in VSM versus pericytes (x axis) and versus ASM (y axis). Genes in the upper right quadrant (violet) are specific to VSM versus both ASM and pericytes, while genes in the lower left quadrant (black) are specifically absent in VSM but not in pericytes nor ASM. (I) Harmonized embedding of VSM from this study (red circles) and overlapping cells from Tabula Muris Senis (gray squares), identified using a rigidly expanded principle component analysis (PCA) ellipse. These cells are originally annotated as pericytes, myofibroblasts, or fibroblasts. (J) Differential expression of select transcription factors in newly identified atlas VSM versus atlas pericytes (x axis) and versus atlas myofibroblasts (y axis).
