Figure 2
Contextual fear following social defeat stress. (A) Experimental design for the testing paradigm utilizing 7 days of social defeat and 3 days of contextual fear testing. (B–C). Protocol for contextual fear training (B) and extinction and extinction retention testing (C), leading to robust fear expression in response to the contextual cue. (D) Latencies to social defeat showing splits exhibited by animals tested in this experiment. (E–H). Total freezing during conditioning averaged individually across the exploration, shock, ITI, or pre-end portion of the training protocol and assessed by one-way ANOVA followed by Tukey post-hoc tests. (I–K). Total freezing expressed as a percent of time spent freezing in 1 min bins over the 8 min testing session for extinction (I, day 9), extinction retention (J, day 10), and persistence of extinction retention (K, day 21). (L–N), Repeated measures analyses of freezing responses separated into 1 min bins, with time as the repeated measure. Performance on each day was tested by a two-way repeated measures ANOVA using time and stress group (control, passive coping, and active coping) as factors). *p < 0.05. Control n = 18, passive coping n = 20, active coping n = 22.
