Figure 1
Experimental design. All individuals from the egg to third instar larvae were kept at 26 °C and 50 ‰ salinity. Third instar larvae were randomly placed into developmental treatments, and trait measures started. In the first treatment, individuals were maintained at constant conditions of temperature (26 ± °C) and salinity (50‰, 50 g/l) throughout the experiment (CC). In the second treatment, individuals were exposed to rising temperature (TC) as follows: third instar larvae were maintained at 29 °C; fourth instar larvae at 31 °C, and pupae at 34 °C. In the third treatment, individuals were exposed to decreasing salinity (SC) from 50‰ to 0‰. Weight and digital pictures were obtained within two hours from the ecdysis.
