Figure 1

Immunomodulating effects of S. aureus derivatives on tumor-infiltrating lymphocytes in TNBC. (A) and (B), 4T1 tumors were injected with > 1 kD molecules from S. aureus spent media (> 1 kD, in red, n = 5) or control media (Con, in black, n = 5). Flow cytometric analysis was performed to determine the percentage of total CD3⁺ T cells, CD8⁺ T cells, and CD4⁺ T cells among viable cells (A) and CD4⁺ T cells and CD8⁺ T cells among total T cells (B). (C) and (D), 4T1 tumors were injected with > 100 kD molecules from S. aureus spent media (> 100 kD, in green, n = 6) or control media (Con, in black, n = 6) (C), and heat-killed S. aureus (killed SA, in blue, n = 4) or control DPBS (Con, in black, n = 4) (D). The percentage of total T cells, CD8⁺ T cells, and CD4⁺ T cells among viable cells were determined for each treatment ((C) and (D)). Data are presented as median with quartiles (truncated violin plots). Unpaired two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.